Effect of oil seed kernel of Azadirachta indica in methanol and ethanol solvents on Tetranychus urticae

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Effect of oil neem seed extract and the neem kernel powder mixed with methanol 85% and ethanol 95% were investigated on two spotted spider mite (Tetranychus urticae) under greenhouse (25 ± 5 °C and 40 ± 10% R. H.) and laboratory conditions. Mortality percent of each above-mentioned solvent in different concertrations (0.03, 0.06, 0.09, 0.12, 0.15, 0.2, 0.3 and 0.5%) by three methods (leaf dip, potted spray tower and direct spraying on infested bean leaves by mite) was determined in a complete randomized design with four replications 24 and 48 hours after treatment. Pooled analysis of variance has been done on data obtained through Abbott formula. Interactions of doses, methods and time on methanol and ethanol solvents were carried out on T. urticae and results indicated that, interaction between doses and solvents statistically found insignificant (P?0.05), but mortality percent increased 48 hours after treatments with stronger doses. Potted spray tower found more effective as compared with other methods which have been applied. The response of bean plants to toxin side effects of neem solvents was variable, which higher doses dried and wilted of bean leaves within a week after applications. The new leaves were also appeared on treated bean plants two to three weeks after treatments.

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