The effects of sucrose and some regulators on freezing tolerance in chickpea studied. Four weeks in vitro explants were cultured in three different MS media without growth regulators and with normal level of sucrose (30 gl-1) (a), without growth regulators and with reduced sucrose (10 gl-1) (b) and medium supplemented with lmg gl-1 BAP and 0.1 mgI1 IBA and with 30 gI-1sucrose (c) for two weeks. The explants were free zed to zero, -4, -8, -12, -16 and -20 C for one hour in programmable freezer after acclimation within 20 days at 4 C. Freezing tolerance determined as lethal temperature (LT50) by probit analysis. The plants cultured in medium (a) had the most freezing tolerance. The in vitro screening of chickpea cultivars in medium (a) would be satisfactory.
Moshiri, F., Bagheri, A. R., & Vesal, S. R. (2003). The effect of medium component on freezing tolerance of three chickpea cultivars. Agriculture (Not Published), 5(2), 51-58.
MLA
Fereshteh Moshiri; Abdol Reza Bagheri; Saeid Reza Vesal. "The effect of medium component on freezing tolerance of three chickpea cultivars", Agriculture (Not Published), 5, 2, 2003, 51-58.
HARVARD
Moshiri, F., Bagheri, A. R., Vesal, S. R. (2003). 'The effect of medium component on freezing tolerance of three chickpea cultivars', Agriculture (Not Published), 5(2), pp. 51-58.
VANCOUVER
Moshiri, F., Bagheri, A. R., Vesal, S. R. The effect of medium component on freezing tolerance of three chickpea cultivars. Agriculture (Not Published), 2003; 5(2): 51-58.
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