This experiment was conducted to investigate the relationship between storing condition of potato seed and application of Giberellic acid (GA3) on quantitative traits and total yield of potato seed tubers. The research was conducted at the Khorram dareh Agro - industry. Three cultivars of potato with two methods of storage and three levels of GA3 (0, 5, 10 ppm) in a factorial design with randomized complete blocks were cultivated. The five ppm of GA3 with storage conditions number two had most effects on potato seed production. The effects of storage conditions in treatments of five and 10 ppm of GA3 on producing amount of main stem didn’t show any significant difference at one percent level. In total, "Marfona" had the highest yield in the most treatment and we have obtained 3.71 kg/m2 on mean weight of tubers from this cultivar.