Genetic manipulation and selection of favorable somaclonal variant from callus culture are supplementary tools for plant breeding. Immature embryos are the most frequently used explant source for in vitro culture. Response of 25 wheat (Triticum aestivum L.) genotypes to callus induction and plant regeneration from immature embryo culture was evaluated under in vitro condition. Basal MS medium supplemented with 2 mg/L 2, 4-D and 0.1 mg/L kinetin was used for callus induction. Callus induction rate and callus growth rate were used as callus induction criteria of cultivars derived immature embryo culture. Response of cultivars to regeneration from callus was evaluated using the percentage and rate of plant regeneration. Average percent of callus production ranged from 46 to 95 percent in "Sholeh" and "Cross Sorkh Tokhm" cultivars, respectively.” Cross Sorkh Tokhm" in view point of callus induction and "Alvand" with considering the regeneration from callus at the shortest time, were ranked as the superior genotypes. Considering the total plants regenerated from the cultured immature embryos, "Alvand", was ranked as the superior genotypes.
Golkar, P., Arzani, K., & Mirmohammadi meibodi, S. A. M. (2007). Callus Induction and Plant Regeneration from Immature Embryos of Wheat Cultivars. Agriculture (Not Published), 9(1), 39-50.
MLA
Pourandokht Golkar; Kazem Arzani; Seyed Ali Mohammad Mirmohammadi meibodi. "Callus Induction and Plant Regeneration from Immature Embryos of Wheat Cultivars", Agriculture (Not Published), 9, 1, 2007, 39-50.
HARVARD
Golkar, P., Arzani, K., Mirmohammadi meibodi, S. A. M. (2007). 'Callus Induction and Plant Regeneration from Immature Embryos of Wheat Cultivars', Agriculture (Not Published), 9(1), pp. 39-50.
VANCOUVER
Golkar, P., Arzani, K., Mirmohammadi meibodi, S. A. M. Callus Induction and Plant Regeneration from Immature Embryos of Wheat Cultivars. Agriculture (Not Published), 2007; 9(1): 39-50.
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