Isolation of Aspergillus flavus from Feeds and Determination of Aflatoxin M1 in Milk from some Dairy Farms of Tehran by HPLC‎‏ ‏

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Isolates of A. flavus obtained from different feeds such as corn, barley, wheat, cotton ‎seed, bread waste, soybean, alfalfa, wheat bran, barley meal, canola meal, corn silage and beet ‎pulp from nine dairy farms in Tehran province during 2004. Ability of sclerotiom production by ‎isolates was tested. Results showed 62.9 percent of isolates produced sclerotiom on CzA medium ‎and 68.5 percent of these isolated were aflatoxigenic. From pollution of feed by A. flavus point of ‎view in dairy farms were between 20.37 - 3.7 percent. 16.67 percent of isolates obtained from ‎beet pulp as the most contaminated feed. Alfalfa, canola meal and corn meal with‏ ‏medium 1.8 ‎percent were of the least in every contaminated feeds. Analysis of milk samples by HPLC ‎showed that the rate of AFM1 in samples varied and ranged from 0.0009 to 0.5269 ppb. ‎Calibration regression coefficients determined 0.99 to 0.997 for these experiments and ‎determined recovery was between 70.31 to 1.4.93 percent in these experiments. ‎

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