عنوان مقاله [English]
نویسندگان [English]چکیده [English]
Cyanogenesis in Prunus species produces cyanogenic glycosides such as monoglycoside Prunasin and digelicosede amygdalin. Despite the mechanism of almond bitterness inheritance has been known, biosynthesis cycle of cyanogenic compounds among different genotypes is still unclear. Total soluble proteins of fruits was extracted from collected sweet and bitter almond genotypes and was studied using western immune blotting assay with Mandelonytril glycosidase and related polyclonal antibody, and products of this enzyme was studied by HPLC. Results showed that all of bitter and sweet genotypes had the ability of enzyme synthesis in the fruit and it is produced since mid stage of fruit development. Only bitter genotypes kept synthesis the enzyme and it was suppressed for sweet ones. The HPLC analysis of metabolites synthesized by the enzyme showed that the amount of cyanogenic content of bitter genotypes was much more than the sweet ones and merely in bitter genotype seeds prunasin content was reduced while amygdalin content was gradually increased. This means bitter genotypes could accumulate amygdalin in their seeds.